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  • Magneto aerotaxis in marine coccoid bacteria ScienceDirect

    MATERIALS AND METHODS. Strain MC 1, a Gram negative magnetotactic coccus (DeLong et al., 1993), was isolated from the OATZ in the Pettasquamscutt estuary in Narragansett. Bay, Rhode Island (Donaghay et al., 1992). Cells were grown in a semi solid medium containing 5 ml of modified Wolfe's mineral solution (.

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  • Magnetosomes Are Cell Membrane Invaginations Organized by the .

    Dec 22, 2005 . Materials and methods: Culture growth conditions. Cultures of Magnetospirillum magneticum sp. AMB 1 were grown in MG medium containing per liter: 5 mL Wolfe's mineral solution (prepared without iron), 0.68 g potassium phosphate, 0.12 g sodium nitrate, 0.07 g sodium acetate, 0.035 g ascorbic acid,.

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  • ATCC ® Medium 2672: Modified Wolfe's Mineral Medium

    The pH of MWMM medium is adjusted to 6.5 to 6.8 by gassing with a mixture of N2 and CO2. The medium is dispensed under a gas phase of 80%. N2 and 20% CO2 into Balch tubes and autoclaved. The vitamin and mineral solutions were added after autoclaving 10µl per 10ml of medium. For growth the headspace is.

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  • Suffocating Congo's War Foreign Policy

    Feb 7, 2015 . In a critique on the campaign to end the trade in conflict minerals from the Democratic Republic of Congo, Foreign Policy contributor Lauren Wolfe asserts . of Congolese civil society, however, reveal critical evidence on why work on conflict minerals is one important part of the solution in eastern Congo.

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  • Trace Mineral Supplement ATCC ® MD TMS

    Feb 2, 2013 . Trace Mineral Supplement (ATCC® MD TMS). Based on Wolfe's formulation. MoreLess. Pricing. For Profit: $65.00; Non Profit: $65.00 . Services. Federal Solutions · Biorepository Management · Deposit Services · Custom Solutions · Testing Services. Standards. Certified Reference Materials (CRMs).

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  • Modified Wolfe's Mineral SolutionWolfe .

    Wolfe's(Wolfe's Mineral Solution Modified)95,,Modified Wolfe's Mineral SolutionWolfe,Modified Wolfe's Mineral Solutio.

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  • Optimization of Magnetosome Production and Growth by the .

    In the bioreactor, cells were grown under anaerobic conditions with N2O in the optimized medium (see details of the choice and exclusion of components and concentrations below), which contained, per liter of artificial seawater (ASW), 33.4 ml modified Wolfe's mineral solution, 41.8 mM sodium succinate, 2.4 mM sodium.

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  • ATCC ® Medium 2672: Modified Wolfe's Mineral Medium

    The pH of MWMM medium is adjusted to 6.5 to 6.8 by gassing with a mixture of N2 and CO2. The medium is dispensed under a gas phase of 80%. N2 and 20% CO2 into Balch tubes and autoclaved. The vitamin and mineral solutions were added after autoclaving 10µl per 10ml of medium. For growth the headspace is.

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  • A highly efficient method for liquid and solid cultivation of the .

    tion was omitted and Wolf's mineral solution was replaced with Balch's trace mineral solution (Balch et al., 1979). The T. maritima basal medium (TMB) contained, per liter, the following: 1.5g KH2PO4; 4.2g Na2HPO4 Á12H2O. (22mM PO4. 3À,); 0.5g NH4Cl; 0.2g MgCl2 Á6H2O (1 mM);. 27g NaCl; 4g yeast extract (Oxoid Ltd,.

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  • MSGM Media Preparation iGEM 2014

    Wolfe's Mineral solution ( Fe). 5 ml. 20 ml. Homemade. The recipe above is to prepare the basic solution of the MG medium. Vitamins and an iron source are missing from this and are added after autoclaving and just before starting cultures. For agar plates: let the medium cool down to ~45°C (hand warm) before adding.

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  • Wolfe's Mineral Solution United States Biological

    Recipe. Add 1.5g nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve. Add the following: 3.0g MgSO4 7H2O; 0.5g MnSO4 H2; 1.0g NaCl; 0.1g FeSO4 7H2O; 0.1g CoCl2 6H2O; 0.1g CaCl2; 0.1g ZnSO4 7H2O; 0.01g CuSO4 5H2O; 0.01g AlK(SO)4 12H2O; 0.01g H3BO3; 0.01g.

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  • MEM Vitamin Solution . Thermo Fisher Scientific

    By adding MEM Vitamin Solution to media, cell growth and viability in culture can be enhanced and biosynthetic burden on cells in vitro can be reduced.Prepared in 0.85% saline. The vitamins in this solution are 100X the concentration in a standard M.

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  • MEM Vitamin Solution . Thermo Fisher Scientific

    By adding MEM Vitamin Solution to media, cell growth and viability in culture can be enhanced and biosynthetic burden on cells in vitro can be reduced.Prepared in 0.85% saline. The vitamins in this solution are 100X the concentration in a standard M.

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  • 1 Techniques for Cultivating Methanogens

    Techniques for Cultivating. Methanogens. Ralph S. Wolfe. Contents. 1. Introduction. 1. 2. Methanogens on a Budget. 2. 2.1. Preparation of oxygen scrubbers. 2. 2.2. .. Prepare a solution of trace minerals (g/L) and add 10 mL of the solution to 1 L of . the Balch stopper on the vial of reducing solution and adjust the pressure.

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  • Trace Mineral Supplement ATCC ® MD TMS

    Feb 2, 2013 . Trace Mineral Supplement (ATCC® MD TMS). Based on Wolfe's formulation. MoreLess. Pricing. For Profit: $65.00; Non Profit: $65.00 . Services. Federal Solutions · Biorepository Management · Deposit Services · Custom Solutions · Testing Services. Standards. Certified Reference Materials (CRMs).

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  • 1337. methylophaga medium 1403 DSMZ

    Mineral Salts Solution: NaCl. 24.0 g. MgCl2 x 6 H2O. 3.0 g. MgSO4 x 7 H2O. 2.0 g. KCl. 0.5 g. CaCl2. 1.0 g. Bis Tris. 0.5 g. Wolfe's Mineral Solution (see below). 10.0 ml. Distilled water. 1000.0 ml. Adjust to pH 8.0 and sterilize by autoclaving. The medium may be solidified by adding. 20.0 g/l agar. Solution T: KH2PO4. 0.7 g.

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  • Wolfe's Mineral Solution United States Biological

    Recipe. Add 1.5g nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve. Add the following: 3.0g MgSO4 7H2O; 0.5g MnSO4 H2; 1.0g NaCl; 0.1g FeSO4 7H2O; 0.1g CoCl2 6H2O; 0.1g CaCl2; 0.1g ZnSO4 7H2O; 0.01g CuSO4 5H2O; 0.01g AlK(SO)4 12H2O; 0.01g H3BO3; 0.01g.

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  • Wolfe's Mineral Solution The Lab Rat

    Add 1.5g nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve. Add the following: 3.0g MgSO4 7H2O 0.5g MnSO4 H2 1.0g NaCl 0.1g FeSO4 7H2O 0.1g CoCl2 6H2O 0.1g CaCl2 0.1g ZnSO4 7H2O 0.01g CuSO4 5H2O 0.01g AlK(SO)4 12H2O 0.01g H3BO3 0.01g Na2MoO4 2H2O.

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  • Modified Wolfe's Mineral SolutionWolfe .

    Wolfe's(Wolfe's Mineral Solution Modified)95,,Modified Wolfe's Mineral SolutionWolfe,Modified Wolfe's Mineral Solutio.

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  • 1337. methylophaga medium 1403 DSMZ

    Mineral Salts Solution: NaCl. 24.0 g. MgCl2 x 6 H2O. 3.0 g. MgSO4 x 7 H2O. 2.0 g. KCl. 0.5 g. CaCl2. 1.0 g. Bis Tris. 0.5 g. Wolfe's Mineral Solution (see below). 10.0 ml. Distilled water. 1000.0 ml. Adjust to pH 8.0 and sterilize by autoclaving. The medium may be solidified by adding. 20.0 g/l agar. Solution T: KH2PO4. 0.7 g.

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  • David Wolfe's Ormus Gold, 50 mL . Longevity Warehouse

    Ormus Gold is a liquid mineral product based on over fifteen years of research by Longevity Warehouse and our research team. Research into Ormus Gold was instigated by independent researcher David Hudson's 1980 rediscovery of unique minerals in Arizonian basalt rock. Hudson called these minerals "monoatomic.

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  • Wolfe's Mineral Solution The Lab Rat

    Add 1.5g nitrilotriacetic acid to approximately 500 ml of water and adjust to pH 6.5 with KOH to dissolve. Add the following: 3.0g MgSO4 7H2O 0.5g MnSO4 H2 1.0g NaCl 0.1g FeSO4 7H2O 0.1g CoCl2 6H2O 0.1g CaCl2 0.1g ZnSO4 7H2O 0.01g CuSO4 5H2O 0.01g AlK(SO)4 12H2O 0.01g H3BO3 0.01g Na2MoO4 2H2O.

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  • Dissimilatory Reduction of Fe(III) and Other Electron Acceptors by a .

    The 10× Wolfe's mineral solution contained (per liter of deionized water) 2.14 g of nitrilotriacetic acid (NTA), 0.1 g of MnCl2 · 4H2O, 0.3 g of FeSO4 · 7H2O, 0.17 g of CoCl2 · H2O, 0.2 g of ZnSO4 · 7H2O, 0.03 g of CuCl2 · 2H2O, 5 mg of KAl(SO4)2 · 12H2O, 5 mg of H3BO4, 0.09 g of Na2MoO4, 0.11 g of NiSO4 · 6H2O, and.

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  • Optimization of Magnetosome Production and Growth by the .

    In the bioreactor, cells were grown under anaerobic conditions with N2O in the optimized medium (see details of the choice and exclusion of components and concentrations below), which contained, per liter of artificial seawater (ASW), 33.4 ml modified Wolfe's mineral solution, 41.8 mM sodium succinate, 2.4 mM sodium.

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  • 1 Distribution of microbial biomass and the potential for anaerobic .

    Dec 2, 2011 . Wolfe's vitamin solution (3), 10 ml L 1 of 10× Wolfe's mineral solution(3), and 10 ml L 1. 175 electron donor mix (13 compounds at 40 mM each substrate:, formate, acetate,. 176 propionate, caprylic acid, methanol, ethanol, n propanol, n butanol, pyruvate, DL malate,. 177 succinate, DL lactate, and glycerol.

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  • Fulvic Acid The Ancient Mineral Health Solution YouTube

    Oct 8, 2011 . Health guru David Wolfe talks on fulvic acid and its amazing health benefits. For UK Europe based people you could look at and anc.

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  • Medium 792 DSMZ

    Solution A: NaCl. 13.85 g. MgSO4 x 7 H2O. 3.50 g. MgCl2 x 6 H2O. 2.75 g. CaCl2 x 2 H2O. 0.38 g. KCl. 0.33 g. NaBr. 0.05 g. H3BO3. 15.00 mg. SrCl2 x 6 H2O solution (0.1% w/v). 7.00 ml. KI solution (0.01% w/v). 0.50 ml. Wolfe's mineral elixir (see next page). 1.00 ml. Na resazurin solution (0.1% w/v). 0.50 ml. Distilled water.

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  • Medium 792 DSMZ

    Solution A: NaCl. 13.85 g. MgSO4 x 7 H2O. 3.50 g. MgCl2 x 6 H2O. 2.75 g. CaCl2 x 2 H2O. 0.38 g. KCl. 0.33 g. NaBr. 0.05 g. H3BO3. 15.00 mg. SrCl2 x 6 H2O solution (0.1% w/v). 7.00 ml. KI solution (0.01% w/v). 0.50 ml. Wolfe's mineral elixir (see next page). 1.00 ml. Na resazurin solution (0.1% w/v). 0.50 ml. Distilled water.

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  • MSGM Media Preparation iGEM 2014

    Wolfe's Mineral solution ( Fe). 5 ml. 20 ml. Homemade. The recipe above is to prepare the basic solution of the MG medium. Vitamins and an iron source are missing from this and are added after autoclaving and just before starting cultures. For agar plates: let the medium cool down to ~45°C (hand warm) before adding.

    get price>>
  • WolfePak Software :: Oil and Gas Accounting Software

    Investors. The accounting solution for investors and mineral owners with growing portfolios that need to track income, expenses and taxes from multiple holdings and different ownership interests. Learn More.

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